Effects of RNA secondary structure on cellular antisense activity

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The influence of antisense oligonucleotide-induced RNA structure on Escherichia coli RNase H1 activity.

The ability of Escherichia coli RNase H1 to hydrolyze structured substrates containing antisense oligonucleotides preannealed to a 47-mer RNA was compared with its ability to hydrolyze unstructured substrates containing antisense oligonucleotides duplexed with 13-mer RNA. These results demonstrate that when antisense oligonucleotides were bound to structured RNA, the resultant duplexes were cle...

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RNA Secondary Structure Prediction.

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ژورنال

عنوان ژورنال: Nucleic Acids Research

سال: 2000

ISSN: 1362-4962

DOI: 10.1093/nar/28.6.1340